Single-cell proteomics reveals cytoplasmic defects in Patl2-deficient oocytes rescued by spindle transfer

The authors investigate whether single-cell proteomics can identify proteins linked to the reduced developmental competence of Patl2-deficient mouse oocytes and explore potential therapeutic interventions for Patl2-related infertility. They find that Patl2-/- oocytes exhibit abnormal protein profiles, which can be rescued by spindle transfer, leading to improved activation and blastocyst rates. This study suggests that spindle transfer may be a viable treatment for infertility associated with PATL2 mutations, addressing cytoplasmic defects identified through proteomic analysis.